Description
Transient gene expression in mammalian cells requires large amounts of high-quality plasmid DNA. As commercially available kits exclusively use single-use columns and have a limited capacity of only 10 mg, InVivo established a proprietary procedure for large-scale DNA preparation. Here, reusable ion exchange columns are used which easily allow the cost-efficient preparation of up to 100 mg plasmid DNA* with a standard size ranging from 1500 bp to 9000 bp. This transfection-grade DNA is animal component-free with a very low level of endotoxins.
*For individual projects, we can also offer larger DNA yields, if required.